Research Article
Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor
Table 1
Primers used for RT-PCR and qPCR.
| Genes | Forward | Reverse | Size (bp) | Accession number |
| Sca-1 | 5′-CTCTGAGGATGGACACTTCT-3′ | 5′-GGTCTGCAGGAGGACTGAGC-3′ | 404 | NM_001271416 | CD34 | 5′-ATGCAGGTCCACAGGGACACG-3′ | 5′-CTGTCCTGATAGATCAAGTAG-3′ | 220 | NM_001111059 | c-kit | 5′-CCATGTGGCTAAAGATGAAC-3′ | 5′-ACTGCTGGTGCTCGGGTTT-3′ | 318 | NM_001122733 | CD73 | 5′-TCCTGGGCTACGATGCTATG-3′ |
5′-CCACAACCTCACCGCCAAC-3′ | 195 | NM_011851 | CD105 | 5′-AGCCTTACCTCTGGATACCG-3′ | 5′-AACGTCACCTCACCCCTTGT-3′ | 190 | NM_007932 | OCT4A | 5′-TGTGGACCTCAGGTTGGACT-3′ | 5′-CTTCTGCAGGGCTTTCATGT-3′ | 201 | NM_013633 | Sox-2 | 5′-GCACATGAACGGCTGGAGCAACG-3′ | 5′-TGCTGCGAGTAGGACATGCTGTAGG-3′ | 206 | NM_011443 | Nanog | 5′-GAGTGTGGGTCTTCCTGGTC-3′ | 5′-GAGGCAGGTCTTCAGAGGAA-3′ | 182 | NM_028016 | Pax6 | 5′-TGCCCTTCCATCTTTGCTTG-3′ | 5′-TCTGCCCGTTCAACATCCTTAG-3′ | 178 | NM_001244200 | Brachyury | 5′-ATGCCAAAGAAAGAAACGAC-3′ | 5′-AGAGGCTGTAGAACATGATT-3′ | 835 | NM_009309 | AFP | 5′-TCGTATTCCAACAGGAGG-3′ | 5′-AGGCTTTTGCTTCACCAG-3′ | 174 | NM_007423 | Tubb5 | 5′-GGAACATAGCCGTAAACTGC-3′ | 5′-TCACTGTGCCTGAACTTACC-3′ | 317 |
NM_011655 |
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