Muscle-Type Specific Autophosphorylation of CaMKII Isoforms after Paced Contractions
Figure 5
Thr287 phosphorylation of CaMKII in glycolytic and oxidative fast muscle compartment after paced contractions in situ. (a) Immunoblot showing the phospho-Thr287-CaMKII signal in stimulated and resting control gastrocnemius medialis muscle (oxidative compartment) after stimulation of the sciatic nerve with a train of 100 pulses of a slow (i.e., 10 Hz) or a fast (i.e., 150 Hz) motor unit-targeted protocol [9]. (b)(c) Bar graphs visualizing the mean + SE of changes in phospho-Thr287-CaMKII levels with stimulation of the oxidative (b) and glycolytic (c) compartment of gastrocnemius medialis. Data reflect combined values from the stimulation 10-Hz and 150-Hz protocol. *denotes versus rest (two-sided paired -test, –14).
