[Retracted] Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

Figure 1

(a) SDS polyacrylamide gel electrophoresis of L. donovani of KMP-11 purified recombinant protein and (b) western blot analysis. (a) SDS polyacrylamide gel electrophoresis of L. donovani of KMP-11 purified recombinant protein. Coomassie brilliant blue staining Lane 1 Marker, Lane 2 CP- Crude Protein, Lane 3 E1 (eluted protein after first washing), Lane 4 E2 (eluted protein after the second washing), Lane 5 E3 (eluted protein after the third washing), Lane 6 F1, Lane 7 F2, Lane 8 F3 (Lane 6-7 protein fraction of KMP-11), and F3 fraction in Lane 8 show the KMP-11 recombinant protein. (b) KMP-11 recombinant protein purified by affinity chromatography, transferred on nitrocellulose paper, and probed with anti-KMP-11 antibody. The arrow indicates the localization of KMP-11 protein. The asterisks show the dimeric and trimeric forms of the KMP-11 protein.