Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities
Figure 2
Fibrinogenolytic activity of Parawixia bistriata venom. Fibrinogen was preincubated with the crude venom of P. bistriata in the presence of divalent ions, EDTA and heparin. The samples were subjected to SDS-PAGE for visualization of protein bands. F = Fibrinogen CV = P. bistriata crude venom. (a) Effect of divalent ions: lanes: (a)1: F (80 μg); (a)2: F + CV (20 μg); (a)3: F + CV + Ca2+ (40 mM); (a)4: F + CV + Co2+ (40 mM); (a)5: F + CV + Fe2+ (40 mM); (a)6: F + CV + Mg2+ (40 mM); (a)7: F + CV + Mn2+ (40 mM); (a)8: F + CV + Na+ (40 mM); (a)9: F + CV + Zn2+ (40 mM). (b) Effect of EDTA and heparin. Lanes: (b)1: F (80 μg); (b)2: F + CV (20 μg); (b)3: F + CV + 10 mM EDTA; (b)4: F + CV + 20 mM EDTA; (b)5: F + CV + 30 mM EDTA; (b)6: F + CV + 10 mM heparin; (b)7: F + CV + 20 mM heparin; (b)8: F + CV + 30 mM heparin.
