Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities

Figure 8

Proteolytic activity of Parawixia bistriata crude venom against the substrate azo-collagen. An increase in absorbance of 0.05 was regarded as 1 unit of enzymatic activity for each milligram of protein. The red bars represent the activity of the native protein and the light gray bars represent the activity of the proteins after incubation with 1 mM EDTA. BJcv: B. jararacussu crude venom; BMcv: B. matogrossensis crude venom. PBcv: P. bistriata crude venom. P1, P2, P3, P4, and P5: fractions from the venom of P. bistriata. Absorbance read at 550 nm.