Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities

Table 2

Nuclear quantification of human lymphocytes treated with Parawixia bistriata spider venom.

Treatments (𝜇g/mL)	% cells/500 cells				CBPI ± S.D.
	^aMono	^bBi	^cTri	^dMulti
Control (+)
6	48.5	38.7	7.6	5.2	1.643 ± 0.1
Control (−)
—	50.3	39	7.3	3.4	1.604 ± 0.07
P. bistriata
2.5	56.8	37.5	3.6	2.1	1.489 ± 0.05
5	50.4	40.2	5.4	4	1.590 ± 0.2
15	53.9	38.6	3.9	3.6	1.536 ± 0.09

aMono: mononucleated, bBi: binucleated, cTri: trinucleated, dMulti: multinucleated. Positive control (+): cisplatin (6 𝜇g/mL). Negative control (−): untreated cells in culture. CBPI: cytokinesis block proliferation index, that defines whether the cultures are multiplying normally after the addition of samples. The following formula was used according to Kirsch-Volders (1997) [6]: CBPI = 1 (mono) + 2 (bi) + 3 (tri + tetra)/500. Mean cells number followed by the standard deviation.