Review Article

Mesenchymal Stem Cells for Regenerative Therapy: Optimization of Cell Preparation Protocols

Figure 2

Protocols and materials used for MSC isolation and expansion. Different methods and materials were used in the recent MSC-therapy clinical trials, in terms of method for bone marrow (BM) preparation for MSC isolation (a), culture flask used for MSC isolation (b), culture medium used for MSCs expansion (c), and serum used for MSC expansion (d). PL: platelet lysate; FBS: fetal bovine serum.
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(a) BM preparation
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(b) Flask for isolation
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(c) Expansion medium
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(d) Serum for expansion