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BioMed Research International
Volume 2014, Article ID 951937, 10 pages
http://dx.doi.org/10.1155/2014/951937
Research Article

Endometrial Receptivity Profile in Patients with Premature Progesterone Elevation on the Day of hCG Administration

1CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, 34295 Montpellier, France
2INSERM U1040, Hôpital Saint-Eloi, 34295 Montpellier, France
3Université Montpellier 1, UFR de Médecine, Equipe “Développement Embryonnaire Précoce et Cellules Souches Embryonnaires Humaines”, 34000 Montpellier, France
4OVO Fertility, 8000 Boulevard Decarie No. 100, Montréal, QC, Canada H4P 2S4
5CHU Montpellier, ART/PGD Division, Département de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, 34295 Montpellier, France
6Laboratoire Dynabio, Polyclinique du Cotentin, 50120 Equeurdreville, France
7CHU Montpellier, Hôpital Gui de Chauliac, Service Anatomie Cytologie Pathologiques, 34295 Montpellier, France
8CHU Léonard de Vinci-Université Paris XIII, Service de Médecine de la Reproduction, Hôpital Jean Verdier, 93143 Bondy, France

Received 16 December 2013; Revised 3 April 2014; Accepted 3 April 2014; Published 28 April 2014

Academic Editor: Javeed Iqbal

Copyright © 2014 Delphine Haouzi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The impact of a premature elevation of serum progesterone level, the day of hCG administration in patients under controlled ovarian stimulation during IVF procedure, on human endometrial receptivity is still debated. In the present study, we investigated the endometrial gene expression profile shifts during the prereceptive and receptive secretory stage in patients with normal and elevated serum progesterone level on the day of hCG administration in fifteen patients under stimulated cycles. Then, specific biomarkers of endometrial receptivity in these two groups of patients were tested. Endometrial biopsies were performed on oocyte retrieval day and on day 3 of embryo transfer, respectively, for each patient. Samples were analysed using DNA microarrays and qRT-PCR. The endometrial gene expression shift from the prereceptive to the receptive stage was altered in patients with high serum progesterone level (>1.5 ng/mL) on hCG day, suggesting accelerated endometrial maturation during the periovulation period. This was confirmed by the functional annotation of the differentially expressed genes as it showed downregulation of cell cycle-related genes. Conversely, the profile of endometrial receptivity was comparable in both groups. Premature progesterone rise alters the endometrial gene expression shift between the prereceptive and the receptive stage but does not affect endometrial receptivity.