Assessment of Mycobacterium bovis Deleted in p27-p55 Virulence Operon as Candidate Vaccine against Tuberculosis in Animal Models

Figure 2

Protection assays in animal models. Organ bacillary loads after intratracheal challenge with M. bovis (a): BALB/c mice were vaccinated with the MbΔp27-p55 or BCG and compared with control nonvaccinated animals. CFUs were determined after 30 days of challenge. Organ bacillary loads (b) and histopathology (c) after aerosol challenge with M. tuberculosis: guinea pigs were vaccinated with the MbΔp27-p55 or BCG and compared with control nonvaccinated animals. Histopathology and CFUs were determined after 30 days of challenge. Data in (a) and (b) were analyzed using a two-tailed unpaired t-test, and data in (c) were analyzed using Mann-Whitney test (^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001).

(a)

(b)

(c)