Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.

Figure 3

The methanol fraction of Solanum integrifolium (ME) inhibits LPS-induced mRNA expression of proinflammatory cytokines and chemokines in RAW264.7 macrophages. RAW264.7 macrophages were cultured with vehicle (0.1% DMSO) or LPS (10 ng/mL) in the presence of the indicated concentrations of methanol fraction (ME) in 6-well plates for 12 h. Total RNA was prepared and the mRNA levels of IL-1β, IL-6, CCL2/MCP-1, and CCL3/MIP1α were detected by RT-Q-PCR, as described in Section 2. Data represent the mean ± SD of three independent experiments. ^∗∗𝑃<0.01 represents significant differences compared with the vehicle control (without LPS). ^##𝑃<0.01 represents significant differences compared with the LPS-treated vehicle.

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