Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.
Figure 4
Involvement of HO-1 upregulation in the anti-inflammatory effect of the methanol fraction of Solanum integrifolium (ME). (a) RAW264.7 macrophages were cultured with vehicle (0.1% DMSO) or LPS (10 ng/mL) in the presence of the indicated concentrations of methanol fraction (ME) in 6-well plates for 12 h. Total RNA was prepared and HO-1 mRNA level was detected by RT-Q-PCR, as described in Section 2. Data represent the mean ± SD of three independent experiments. represents significant differences compared with the vehicle control (without LPS). ; represent significant differences compared with the LPS-treated vehicle. (b) RAW264.7 cells were cultured as described above for 16 h. Total cell lysates were prepared and HO-1 protein expression was detected by Western blotting, as described in Section 2. These blots are representative ones from one of three independent experiments. (c) RAW264.7 cells were treated with ME (0.1 mg/mL) and LPS (10 ng/mL) in the presence or absence of ZnPP (10 μM) for 20 h, and the amount of NO produced in the medium was determined by the Griess reaction. Data were obtained from four independent experiments and are expressed as the mean ± SD. indicates significant differences from the respective LPS-treated group; indicates significant differences from Znpp untreated group.
