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BioMed Research International
Volume 2014 (2014), Article ID 956579, 10 pages
Research Article

Effects of Dental Methacrylates on Oxygen Consumption and Redox Status of Human Pulp Cells

1Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy
2Laboratorio di Patologia Clinica, Ospedale M.G. Vannini, Via dell’Acqua Bullicante, 00177 Rome, Italy
3Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples “Federico II”, Via G. Pansini 5, 80131 Napoli, Italy
4Istituto di Chimica del Riconoscimento Molecolare, C.N.R., c/o Largo Francesco Vito 1, 00168 Rome, Italy
5Istituto di Clinica Odontoiatrica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy

Received 24 April 2013; Revised 13 November 2013; Accepted 22 November 2013; Published 12 February 2014

Academic Editor: Chiu-Chung Young

Copyright © 2014 Giuseppina Nocca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Several studies have already demonstrated that the incomplete polymerization of resin-based dental materials causes the release of monomers which might affect cell metabolism. The aim of this study was to investigate the effects of triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, urethane dimethacrylate, and 2-hydroxyethyl methacrylate on (1) cellular energy metabolism, evaluating oxygen consumption rate, glucose consumption, glucose 6-phosphate dehydrogenase activity, and lactate production, and (2) cellular redox status, through the evaluation of glutathione concentration and of the activities of enzymes regulating glutathione metabolism. Methods. Human pulp cells were used and oxygen consumption was measured by means of a Clark electrode. Moreover, reactive oxygen species production was quantified. Enzymatic activity and glucose and lactate concentrations were determined through a specific kit. Results. Triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and 2-hydroxyethyl methacrylate induced a decrease in oxygen consumption rate, an enhancement of glucose consumption, and lactate production, whilst glucose 6-phosphate dehydrogenase and glutathione reductase activity were not significantly modified. Moreover, the monomers induced an increase of reactive oxygen species production with a consequent increase of superoxide dismutase and catalase enzymatic activities. A depletion of both reduced and total glutathione was also observed. Conclusion. The obtained results indicate that dental monomers might alter energy metabolism and glutathione redox balance in human pulp cells.