Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico

Table 1

PCR primer for each virulence factor. Primer sequence was taken from Johnson and Stell, 2000 [5] and Blackburn et al., 2009 [17].

Genes	Primer (5′-3′)
ecpA	TGA AAA AAA AGG TTC TGG CAA TAG C
	CGC TGA TGA GGA GAA AGT GAA
ecpRB	GTC ACA TGG CAA AAT GAT TAC AGC
	TCA CGG GAA TGA ACT TAT CAC CC
papC	GTG GCA GTA TGA GTA ATG ACC GTT A
	ATA TCC TTT CTG CAG GGA TGC AAT A
sfaS	GTG GAT ACG ACG ATT ACT GTG
	CCG CCA GCA TTC CCT GTA TTC
focG	CAG CAC AGG CAG TGG ATA CGA
	GAA TGT CGC CTG CCC ATT GTC
fimH	TGC AGA ACG GAT AAG CCG TGG
	GCA GTC ACC TGC CCT CCG GTA
sfa/fogDE	CTC CGG AGA ACT GGG TGC ATC TTA C
	CGG AGG AGT AAT TAC AAA CCT GGC A
cnf1	AAG ATG GAG TTT CCT ATG CAG GAG
	CAT TCA GAG TCC TGC CCT CAT TAT T
hlyA	AAC AAG GAT AAG CAC TGT TCT GGC T
	ACC ATA TAA GCG GTC ATT CCC GTC A
cdt-s	GAA AGT AAA TGG AAT ATA AAT GTC CG
	GAA AAT AAA TGG AAC ACA CAT GTC CG
cdt-a	AAA TCA CCA AGA ATC ATC CAG TTA
	AAA TCT CCT GCA ATC ATC CAG TTT A
colV	CAC ACA CAA ACG GGA GCT GTT
	CTT CCC GCA GCA TAG TTC CAT
fyuA	TGA TTA ACC CCG CGA CGG GAA
	CGC AGT AGG CAC GAT GTT GTA
iutA	GGC TGG ACA TCA TGG GAA CTG G
	CGT CGG GAA CGG GTA GAA TCG
ibeA	AGG CAG GTG TGC GCC GCG TAC
	TGG TGC TCC GGC AAA CCA TGC
Rfc	ATC CAT CAG GAG GGG ACT GGA
	AAC CAT ACC AAC CAA TGC GAG
traT	GGT GTG GTG CGA TGA GCA CAG
	CAC GGT TCA GCG ATC CCT GAG