Figure 7: Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total FOXO3a (tFOXO3a) and phosphorylated FOXO3a (P-FOXO3a) ((a) and (c)), total mTOR (t-mTOR) and phosphorylated (P-mTOR) ((b) and (d)), and P-FOXO3a/tFOXO3a and P-mTOR/tmTOR ratios ((e) and (f), resp.). Bands representing tFOXO3a, P-FOXO3a, t-mTOR, and P-mTOR are illustrated in (g). α-Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group (), DX = dexamethasone group (), n-3 = n-3 PUFA group (), and DX + n-3 = dexamethasone + n-3 PUFA group (). Values represent means ± S.E.M., analyzed by Student’s t-test, ^{#}.