Cigarette Smoke-Exposed Candida albicans Increased Chitin Production and Modulated Human Fibroblast Cell Responses
CSC-pretreated C. albicans adhered more to gingival fibroblasts. C. albicans was incubated for 24 h in the presence of CSC and then used to infect gingival fibroblast cultures for 3 or 6 h. Each culture condition was photographed following crystal violet staining (panel (a), photos after 6 h). Adherent C. albicans colonies were counted under an inverted microscope and plotted (panel (b), ). Significance was obtained by comparing the CSC-pretreated and untreated C. albicans.
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