Cigarette Smoke-Exposed Candida albicans Increased Chitin Production and Modulated Human Fibroblast Cell Responses

Figure 8

Growth of CSC-pretreated C. albicans following contact with gingival fibroblasts. C. albicans was incubated for 24 h in the presence of CSC and then used to infect gingival fibroblast cultures for 24 or 48 h at 37°C. The cultures were performed in a transwell culture system allowing for indirect contact between C. albicans and the fibroblasts. C. albicans cells were then collected and subjected to an MTT colorimetric assay ().