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BioMed Research International
Volume 2014 (2014), Article ID 964614, 10 pages
Research Article

Female Aging Alters Expression of Human Cumulus Cells Genes that Are Essential for Oocyte Quality

1UFR de Médecine, Université Montpellier 1, 34295 Montpellier, France
2CHU Montpellier, Institut pour la Médecine Régénérative et Biothérapies, Hôpital Saint-Eloi, INSERM U1040, 34295 Montpellier, France
3ART-PGD Department, CHU Montpellier, Hôpital Arnaud de Villeneuve, 34295 Montpellier, France
4Institute of Molecular Genetics of Montpellier, 34293 Montpellier, France

Received 2 July 2014; Revised 15 July 2014; Accepted 17 July 2014; Published 3 September 2014

Academic Editor: Calvin Yu-Chian Chen

Copyright © 2014 Tamadir Al-Edani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material available online includes: (i) Sequence of the forward and reverse primers used for the qRT-PCR analysis. (ii) List of the 1,874 genes whose differential expression in the three age groups was significant (iii) List of the 60 genes (20 for each age category) used for the hierarchical clustering. (iv) exhaustive lists of miRNAs that are putative regulators of genes over-expressed in CCyounger, CCmedian, and CColder, retrieved by GenGo. (v) GenGo predicted miRNAs that target some significant genes implicated in inflammatory response, angiogenesis, insulin and TGF-β signaling pathways. (vi) qRT-PCR validation on independent cohorts of individual CCs of gene members of the key pathways that are discussed in the manuscript with the addition of a 4th age category (age: 35-36 years).

  1. Supplementary Material