Liquiritigenin Induces Tumor Cell Death through Mitogen-Activated Protein Kinase- (MPAKs-) Mediated Pathway in Hepatocellular Carcinoma Cells

Figure 3

LQ caused the dissipation of Δ𝜓𝑚 and abnormal expression of Bcl-2 and Bcl-xL in hepatocellular carcinoma cells. (a) LQ (200–400 μM) significantly caused the dissipation of Δ𝜓𝑚 which was detected by JC-1 staining. Qualification data were expressed as the ratio of red to green fluorescent intensity. (b) LQ (400 μM) time-dependently reduced the expressions of Bcl-2 and Bcl-xL from 6 h to 24 h. Quantification data of the expressions of Bcl-2 and Bcl-xL were normalized by corresponding GAPDH and expressed as percent of corresponding control cells. Data are expressed as mean ± S.D. (𝑛=3) and analyzed using one-way ANOVA. *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 versus control cells.

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