Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept
Figure 4
Shake-flask approach: expression analysis and binding capability. Expression of 48scFv (a) and 48scFvSCR1920 (b) in X33 cells was tested in shake flasks under different conditions and subsequently tested for binding to MuLV-env on Mus dunni cells using flow cytometry (c). For FACS-analysis 48scFv-constructs were derived from 0,5% methanol fed-cultures. 10 µL from culture SN was loaded directly for SDS-PAGE analysis. MetOH: methanol; CA: casamino acids; Gly: glycerol. Controls for FACS-analysis, see Supporting Information S1 available online at http://dx.doi.org/10.1155/2014/971345.
