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BioMed Research International
Volume 2014, Article ID 971857, 7 pages
Research Article

O18Quant: A Semiautomatic Strategy for Quantitative Analysis of High-Resolution 16O/18O Labeled Data

1Center for Quantitative Sciences, Vanderbilt University, Nashville, TN 37027, USA
2Center for Proteomics and Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
3Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200031, China

Received 28 February 2014; Accepted 18 April 2014; Published 11 May 2014

Academic Editor: Leng Han

Copyright © 2014 Yan Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The protein ATP5I RAT with the highest ratio of 65.8 in a 2:1 sample was chosen to validate if that ratio was correct.

Two peptides were quantified in protein ATP5I RAT with respective ratios of 3.09 and 50. The questionable one with sequence R.YSYLKPR.A and ratio 50 was validated at individual scan level. The peptide and protein ratio became reasonable after manual validation and correction.

  1. Supplementary Figures