Targeting the Neddylation Pathway to Suppress the Growth of Prostate Cancer Cells: Therapeutic Implication for the Men’s Cancer
Figure 2
MLN4924 inhibited prostate cancer cell growth. (a) Morphological observations of MLN4924-treated cells. Cells were treated with indicated concentrations of MLN4924 for 48 hours and then photographed. (b) MLN4924 inhibited the proliferation of prostate cancer cells. Cells were seeded in 96-well plates in triplicate and treated with indicated concentrations of MLN4924 for 96 hours; cell viability was determined by ATPlite kit (, ). (c) MLN4924 inhibited clonogenic cell survival of prostate cancer cells. DU145 and PC3 cells were seeded into 60 mm dishes in duplicate and then grown in the presence or absence of MLN4924 for 12 days. The colonies with more than 50 cells were counted, following crystal violet staining (, ).
