Targeting the Neddylation Pathway to Suppress the Growth of Prostate Cancer Cells: Therapeutic Implication for the Men’s Cancer
Figure 4
MLN4924 induced G2 phase cell cycle arrest and apoptosis in prostate cancer cells. Subconfluent cells were treated with MLN4924. Forty-eight hours later, one portion of cells was used for cell cycle profile analysis (a), while the other portion was subjected to IB analysis ((b) and (c)). (a) MLN4924 induced G2 phase cell cycle arrest. Cell percentages at G2 phase were 5%, 7.5%, and 46% respectively, when treated with MLN4924 at 0, 0.1 and 0.33 μM. (b) IB analysis to determine the expression of WEE1 and p-histone H3. (c) MLN4924 induced apoptosis in prostate cancer cells. Cells were treated with MLN4924 for 48 hours, followed by IB analysis using antibodies against c-Caspase-3 and c-PARP with GAPDH as a loading control.
