Association of CHMP4B and Autophagy with Micronuclei: Implications for Cataract Formation

Figure 2

CHMP4B localizes to chromosome bridges and micronuclei. (a), (b) Confocal micrographs of HeLa cells stained with CHMP4B, Aurora B, and Hoechst. CHMP4B associates with chromosome bridges (arrows) (scale bars: 10 μm) (a) as also presented in higher magnification (arrows) (scale bars: 10 μm) (b). (c), (d) Confocal micrographs of HeLa cells, stained with CHMP4B and Hoechst. CHMP4B attaches to chromosome bridges (arrows) (scale bars: 5 μm) (c) and colocalizes with the DNA present in the chromosome bridge (arrows) (scale bars: 10 μm) (d). (e) Confocal micrographs of HeLa cells stained with CHMP4B and Hoechst. CHMP4B localizes to the micronucleus (arrow). Scale bars: 10 μm.