Association of CHMP4B and Autophagy with Micronuclei: Implications for Cataract Formation

Figure 3

CHMP4B co-mmunoprecipitates with Histone 2B and Lamin A. (a) Confocal micrographs of HeLa cells stained with CHMP4B, Histone 2B, and DAPI. CHMP4B colocalizes with Histone 2B in the micronucleus (arrow). Magnification of the micronucleus is shown in the inset. Scale bars: 10 μm. (b)–(d) Confocal micrographs of HeLa cells stained with CHMP4B, Lamin A, and DAPI. CHMP4B colocalizes in some cases with Lamin A in the micronucleus (arrow) (b) and in some cases does not colocalize with Lamin A (arrow) (c) and is localizing to the chromosome bridge stained by Lamin A (arrows) (d). Scale bars: 10 μm. (e) HeLa cell lysates were subjected to immunoprecipitation (IP) with an antibody against CHMP4B. Immunoprecipitated proteins were detected by Western blotting, using anti-H2B, anti-Lamin A, and anti-CHMP4B antibodies.