Association of CHMP4B and Autophagy with Micronuclei: Implications for Cataract Formation
Figure 5
CHMP4B-mutant found in cataract shows reduced localization to chromosome bridges and micronuclei. (a)-(b) Confocal micrographs of HeLa cells transfected with FLAG-CHMP4B full length and stained with FLAG epitope M2 antibody and Hoechst. FLAG-CHMP4B full length associates strongly with chromosome bridges and micronuclei (a)-(b) (arrows). Scale bars: 10 μm and 5 μm. (c)-(d) Confocal micrographs of HeLa cells transfected with FLAG-D129V-CHMP4B full length and stained with FLAG epitope M2 antibody and Hoechst. FLAG-D129V-CHMP4B full length does not associate strongly with chromosome bridges and micronuclei (arrows). Scale bars: 10 μm. (e) Graphic presentation of quantification of %CHMP4B colocalization with DNA in transfected cells with FLAG-CHMP4B full length wild type versus FLAG-CHMP4B-D129V full length mutant. Error bars show mean ± SD. FLAG-CHMP4B full length: 5 independent experiments, cells. FLAG-CHMP4B-D129V full length: 5 independent experiments, cells. value for full length FLAG-constructs: 0.048. The values were derived from comparing means by independent samples -test (SPSS, v.16.0).
