Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases : A Biotechnological Tool to Improve the Production of Antibodies

Figure 1

Electrophoretic and immunochemical analysis of the antigen-antibody reaction. (a) SDS-PAGE with reducing agents: lane 1, BthTX-I; lane 2, BthTX-II; lane 3, BjussuCV; lane 4, alk-BthTX-I; lane 5, alk-BthTX-II; lane 6, aprotinin (9 kDa); lane 7, α-lactalbumin (14 kDa); lane 8, carbonic anhydrase (30 kDa), and albumin egg (45 kDa). Antigen immunodiffusion: (b) native BthTX-I (central well) against its native anti-BthTX-I (1, 2, and 3 wells) and BPB-modified (4, 5, and 6 wells) antibodies; (c) native BthTX-II (central well) against its native anti-BthTX-II (1, 2, and 3 wells), and BPB-modified (4, 5, and 6 wells) antibodies. The precipitation lines were achieved using immunodiffusion gel in a wet chamber at 25°C for 48 h. Antigen immunoelectrophoresis: (d) anti-alk-BthTX-I (anti-BthTX-I-BPB) (central well) against its BthTX-I (1) and alk-BthTX-I (2) antigens; (e) anti-alk-BthTX-II (anti-BthTX-II-BPB) antibodies (central well) against its BthTX-II (3) and alk-BthTX-II (4) antigens. Results are representative of experiments carried out in triplicate.