Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases : A Biotechnological Tool to Improve the Production of Antibodies

Table 1

Influence of BjussuCV, BthTX-I, BthTX-II, alk-BjussuCV, alk-BthTX-I, and alk-BthTX-II on LD₅₀ and phospholipase A₂ (PLA₂) activity.

Sample	LD50 (mg/kg)	PLA2^∗ (U/mg)
BjussuCV	5.7 ± 0.9	71.9 ± 1.1
Alk-BjussuCV	5.8 ± 0.8	Residual
BthTX-I	8.0 ± 0.9	0.0
Alk-BthTX-I	40.0 ± 1.2	0.0
BthTX-II	7.5 ± 0.8	37.1 ± 1.2
Alk-BthTX-II	37.3 ± 1.1	0.0

∗Measured through potentiometric titration [37].