Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases : A Biotechnological Tool to Improve the Production of Antibodies

Table 2

Neutralization of edema, PLA₂ activity, and 2x LD₅₀ induced by the respective antibodies of native and modified forms of B. jararacussu crude venom and isolated toxins.

Venom or isolated PLA2	Neutralization of 2 × LD^∗50		Neutralization of edema	Neutralization of PLA2 activity
	Antibodies		Antibodies	Antibodies
	Native	Alkylated	Native	Native	Alkylated
BjussuCV	100%	100%	30%	80%	50%
BthTX-I	100%	83%	5%	—	—
BthTX-II	100%	83%	15%	55%	25%

∗LD₅₀ assay was done after previous incubation of the venom or toxin with its respective antibody (1 : 50, w/w) for 30 min at 37°C. Results express the percentage of animal survival.