Advanced Heat Map and Clustering Analysis Using Heatmap3

Figure 1

An example of “heatmap3” package. The heat map was generated based on 30 samples from TCGA BRCA dataset. The dendrogram of samples (top) was divided into two parts based on the correlation between samples’ gene expression and then labeled, respectively. The categorical annotation bars (above heat map) demonstrate the annotation for age, TN, HER2, PR, and ER. The color bar on the left side demonstrates the log2 fold changes and negative log10 𝑃 values from comparison of triple negative patients versus nontriple negative patients.