Huangqin-Tang Ameliorates TNBS-Induced Colitis by Regulating Effector and Regulatory CD4⁺ T Cells

Figure 3

HQT regulates frequencies of Th1, Th2, Th17, and Treg in the TNBS-induced colitis model. Rats with TNBS-induced colitis were treated with or without HQT (120 mg/kg) and analyzed 7 days after treatment. Lamina propria mononuclear cells (LPMCs) were isolated from each group and subjected to intracellular IFN-γ, IL-4, IL-17, and Foxp3 staining. (a) The frequency of T helper 1 (Th1) (CD4⁺IFN-γ⁺), Th2 (CD4⁺IL-4⁺), Th17 (CD4⁺IL-17⁺), and regulatory T (Treg) (CD4⁺CD25⁺Foxp3⁺) was determined by flow cytometry. Numbers represent the percentages of IFN-γ, IL-4, IL-17A-expressing CD4⁺ T cells, and Foxp3-expressing CD4⁺CD25⁺ T cells in each quadrant. (b) Quantitative analysis of the frequency and total number of Th1, Th2, Th17, and Treg in LPMCs. Results represent the mean ± SD from eight mice per group. , versus the control group. ^Δ, ^ΔΔ versus TNBS-treated rats.

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