Huangqin-Tang Ameliorates TNBS-Induced Colitis by Regulating Effector and Regulatory CD4⁺ T Cells

Figure 6

HQT regulates protein and mRNA levels of T-bet, GATA-3, ROR-γt, and Foxp3 in the TNBS-induced colitis model. Rats with TNBS-induced colitis were treated with or without HQT (120 mg/kg). (a) Whole colon tissue homogenates collected at 7 days after HQT treatment were examined for T-bet, GATA-3, RORγt, and Foxp3 by western blot analysis. Each lane corresponds to an individual mouse. (b) Distal colons collected at day 7 after HQT treatment were used to isolate RNA for expression analysis of T-bet, GATA-3, ROR-γt, and Foxp3 by real-time PCR. Results represent the mean ± SD from eight mice per group. ^Δ, ^ΔΔ versus TNBS-treated rats.

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