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BioMed Research International
Volume 2015, Article ID 103137, 8 pages
Research Article

Quantification of Osseointegration of Plasma-Polymer Coated Titanium Alloyed Implants by means of Microcomputed Tomography versus Histomorphometry

1Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany
2Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany
3Institute of Physics, University of Greifswald, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany
4Department of Informatics and Electrical Engineering, Institute of Device Systems and Circuit Technology, University of Rostock, Albert-Einstein-Straße 2, 18059 Rostock, Germany
5Department of Oral and Maxillofacial Surgery, University Medical Center Rostock, Schillingallee 35, 18057 Rostock, Germany

Received 11 December 2014; Revised 15 April 2015; Accepted 21 April 2015

Academic Editor: Despina Deligianni

Copyright © 2015 Carolin Gabler et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A common method to derive both qualitative and quantitative data to evaluate osseointegration of implants is histomorphometry. The present study describes a new image reconstruction algorithm comparing the results of bone-to-implant contact (BIC) evaluated by means of µCT with histomorphometry data. Custom-made conical titanium alloyed (Ti6Al4V) implants were inserted in the distal tibial bone of female Sprague-Dawley rats. Different surface configurations were examined: Ti6Al4V implants with plasma-polymerized allylamine (PPAAm) coating and plasma-polymerized ethylenediamine (PPEDA) coating as well as implants without surface coating. After six weeks postoperatively, tibiae were explanted and BIC was determined by µCT (3D) and afterwards by histomorphometry (2D). In comparison to uncoated Ti6Al4V implants demonstrating low BIC of 32.4% (histomorphometry) and 51.3% (µCT), PPAAm and PPEDA coated implants showed a nonsignificant increase in BIC (histomorphometry: 45.7% and 53.5% and µCT: 51.8% and 62.0%, resp.). Mean BIC calculated by µCT was higher for all surface configurations compared to BIC detected by histomorphometry. Overall, a high correlation coefficient of 0.70 () was found between 3D and 2D quantification of BIC. The μCT analysis seems to be suitable as a nondestructive and accurate 3D imaging method for the evaluation of the bone-implant interface.