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BioMed Research International
Volume 2015, Article ID 107149, 11 pages
Research Article

Environmental Enrichment Attenuated Sevoflurane-Induced Neurotoxicity through the PPAR-γ Signaling Pathway

1Department of Anesthesiology, Shanghai East Hospital, Tongji University School of Medicine, No. 150, Jimo Road, Shanghai 200120, China
2Department of Anesthesiology, The Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai Medical College of Fudan University, Shanghai 200031, China

Received 29 January 2015; Revised 16 March 2015; Accepted 25 March 2015

Academic Editor: Sung U. Kang

Copyright © 2015 Yupeng Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

To explore whether the PPARγ target genes are affected by sevoflurane, we test several PPARγ target genes such like AP2,Wnt1, IGF-1,IGFBP7, Il-6 and TNF-α by RT-PCR. Finally, we found that these several PPARγ target genes were dramatically increased at mRNA levels (Supply figure A).Moreover, in order to support their conclusion further, we test if PPARγ antagonist abolishes the effect of environmental enrichment on sevoflurane treatment. The mice were treated with PPARγ antagonist GW 6471(5mg/kg) intraperitoneally before it was put in the EE every day for 2 h from P8–P30. At the end, we found that PPARγ antagonist abolishes the effect of environmental enrichment on Sevoflurane treatment (Supply figure B).

  1. Supplementary Figure