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BioMed Research International
Volume 2015, Article ID 108571, 14 pages
http://dx.doi.org/10.1155/2015/108571
Research Article

In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

1Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany
2Institute for Regenerative Cures, UC Davis, Sacramento, CA 95817, USA
3Department of Biological Sciences, California State University, Sacramento, CA 95819, USA
4Institute of Biomedical Engineering, UCL, The Royal Institution, London W1S 4BS, UK
5FONDAP Center for Genome Regulation, Faculty of Sciences, University of Chile, 7800024 Santiago, Chile

Received 18 February 2015; Revised 2 April 2015; Accepted 9 April 2015

Academic Editor: Robert Gauvin

Copyright © 2015 Elizabeth A. Wahl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.