In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Figure 1

AdMSC characterization. The immune phenotype of the cells was evaluated by labeling cells with Phycoerythrin-conjugated antibodies for flow cytometry. The blue histogram indicates the isotype control (a). In order to evaluate their differentiation potential, AdMSCs were cultured with control (not shown), osteogenic, adipogenic, or chondrogenic medium (b). Calcium precipitation as a result of osteogenic differentiation was confirmed by Alizarin Red S staining (left panel), triglyceride-containing vacuoles emerging from adipogenic differentiation were stained used Oil Red O (middle panel), and cartilaginous glycosaminoglycans and mucins were stained using Alcian Blue with a Nuclear Fast Red nucleic cross stain. Staining was performed after 21 d in culture. Scale bars represent 100 μm. , .

(a)

(b)