In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Figure 5

Secretion profile of AdMSC seeded scaffolds. Human cytokine and angiogenesis arrays were utilized in order to analyze supernatants after a 48 h incubation to detect if there is an effect of the scaffolds interaction with the cells on paracrine factor release. Decellularized dermis scaffolds were excluded as previous data revealed that it did not provide a compatible environment for the cells to migrate and flourish. Chitosan films and collagen-GAG matrices show a decrease in expression of IL-8 in comparison to fibrin matrices, which is similar to two-dimensional conditions. Collagen-GAG matrices had a significant release of IL-6, while chitosan films had an increase of Serpin E1 release over all other conditions. There are significant differences in release of PIGF and VEGF from all scaffolds in comparison to two-dimensional cultures. ; ; ; when compared to 2D control. , .