Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells

Figure 2

Pathway analysis of human fibroblasts and hOF-iPSCs. (a) Gene expression of ESCs markers and reprogramming factors. The numbers indicate average signal values in each cell type. Red: highly expressed genes in hOFs and hDFs compared with hiPSCs. (b) and (c) Pathways with low (b) and high (c) expression in human fibroblasts compared with those in hOF-iPSCs. Numbers indicate enrichment scores provided by DAVID. The top three clusters are colored. Blanks indicate “not listed” in the samples. The top three clusters are highlighted in blue (b) and in red (c), respectively. (d) A diagram of various GSL-biosynthetic pathways. Red and blue colors indicate genes with high and low expressions in human fibroblasts, respectively. cer: ceramide; Gb with subscript: globoside with the number of carbohydrates; G with subscript: ganglioside with subclass; Lc with subscript: lacto- with the number of carbohydrates; nLc-: neolacto-; Fuc: fucose; GalNAc: N-acetylgalactosamine; NeuAc: N-acetylneuraminic acid. (e) Individual gene-expression levels of each GSL-biosynthetic pathway in hOFs, hDFs, and hOF-iPSCs. Red and Blue indicate the same as in (d).