Gene Knockout Identification Using an Extension of Bees Hill Flux Balance Analysis

Table 3

Comparison between different methods for production of Succinic acid by E. coli.

Method	Growth rate (1/hr)	BPCY	List of knockout genes
BHFBA	0.7988	0.93656	PTAr, RPE, SUCD1i
BAFBA [9]	0.62404	0.66306	FUM, PTAr, TPI
SA + FBA [5]	N/A	0.39850	ACLD19, DRPA, GLYCDx, F6PA, TPI, LDH_D2, EDA, TKT2, LDH_D-
OptKnock [3]	0.28	N/A	ACKr, PTAr, ACALD

Note. The bold numbers represent the best result. N/A: not applicable. Common genes in either 2 methods. BPCY is in gram (gram-glucose·hour)⁻¹.