(a) Control
(b) Six h after neutron radiation of 4Gy
(c) Twenty-four h after neutron radiation of 4Gy
(d) Six h after neutron radiation of 4Gy with LY294002 treatment
(e) Twenty-four h after neutron radiation of 4Gy with LY294002 treatment
Figure 1: The changes of IEC-6 appearance (IPCM, magnification of 200x). (a) Control, the IEC-6 cells looked like applanatus polygon or fusiform shape and aggregated together shape of chrysanthemum thyse appearance. (b) Six h after neutron radiation of 4Gy, the IEC-6 cells swelled and became approximately round shaped and lots of dead cells floated on the culture solution. (c) Twenty-four h after neutron radiation of 4Gy, the cells were injured more seriously than those of 6 h after neutron irradiation. ((d), (e)) Neutron irradiation of 4Gy with LY294002 treatment group cells, the cells were injured more seriously than those of the neutron irradiation group.