In Vivo Targeted Magnetic Resonance Imaging of Endogenous Neural Stem Cells in the Adult Rodent Brain

Figure 9

Histologic assessment of the distribution of SPIONs. At 1 day and 3 days after injection, -weighed images show hypointense signal in the SVZ and the beginning of RMS (arrows) in an animal injected with anti-CD15-SPIONs ((a), (b)). The corresponding DAB-enhanced Prussian blue staining reveals that anti-CD15-SPIONs are localized around cells and extracellular matrix (arrows) within SVZ ((c), (d)) and RMS (e) at 3 days after injection, which is well matched with MRI findings. In an animal that received nontargeted SPION injection, no hypointense signal is present in the SVZ and RMS ((f), (g)). DAB-enhanced Prussian blue staining reveals that no iron oxide nanoparticles were found in the SVZ or RMS. Bars in (c) and (h) equal 100 μm; bars in (d), (e), (i), and (j) equal 50 μm.

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