Research Article
Ki-67 Expression in CRC Lymph Node Metastasis Does Not Predict Survival
Table 1
Detailed aspects of the immunohistochemical procedure used to visualise the Ki-67.
| Protein marker | Antigen retrieval |
Peroxidase inactivation | Detection system | Antibody | Company | Dilution | Incubation period |
| Ki-67 | Citrate Buffer 0.01 M pH = 6.0 | 3% H2O2 in methanol, 10 min. | RTU Vectastain ABC elite Reagent | GenNova | 1 : 200 | Overnight |
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