Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry

Figure 1

Two-dimensional gel electrophoresis-based Western blot analysis of antiphosphotyrosine proteins in a glioblastoma tissue (160 μg protein per 2D gel). (a) Silver-stained image on a 2D gel before transfer of proteins to a PVDF membrane. (b) Silver-stained image on a 2D gel after transfer of proteins to a PVDF membrane. (c) Western blotting image of antiphosphotyrosine proteins (antiphosphotyrosine antibodies + secondary antibody). (d) Negative control of Western blotting to show the cross-reaction of the secondary antibody (only the secondary antibody, no antiphosphotyrosine antibody).

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