Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry

Table 1

Putative phosphotyrosine-containing proteins identified with 2DGE-based Western blotting and tandem mass spectrometry.

Spot number	Swiss-Prot number	Protein name	Molecular weight	pI	Mascot score	Number of matched unique peptides	Predicted Tyr-phosphomotif	Tyr-phosphomotif position
1	P07900	Heat shock protein HSP 90-alpha	85006	4.94	258	5	KhndDeqY; KklsEllrY; KhglEviY	153–160; 457–465; 513–520
1	P08238	Heat shock protein HSP 90-beta	83554	4.97	237	5	KhndDeqY; RrlsEllrY; KrgfEvvY	148–155; 449–457; 505–512
1	Q12931	Heat shock protein 75 kDa, mitochondrial	80345	8.3	138	1	RhlaEhspY	505–513
2	P08107	Heat shock 70 kDa protein 1A/1B	70294	5.48	52	2	RmvqEaekY	517–525
12	Q71U36	Tubulin alpha-1A chain	50788	4.94	1591	12	KsklEfsiY	164–172
12	P68363	Tubulin alpha-1B chain	50804	4.94	1521	12	KsklEfsiY	164–172
12	Q9NY65	Tubulin alpha-8 chain	50746	4.94	689	7	KsklEfaiY	164–172
18, 27	P60709	Actin, cytoplasmic 1	42052	5.29	138	2	KiltErgY; RdikEklcY	191–198; 210–218
27	Q562R1	Beta-actin-like protein 2	42318	5.39	105	2	KiltErgY; RdvkEklcY	192–199; 211–219
21, 38, 47, 48, 51	P14136	Glial fibrillary acidic protein	49907	5.42	1331	8	RhvrEaasY	316–324
30	P07195	L-lactate dehydrogenase B chain	36900	5.71	130	4	KivaDkdY; KmvvEsaY	77–84; 233–240
32, 33	P62258	14-3-3 protein epsilon	29326	4.63	223	4	KmkgDyhrY	123–131
34	P08758	Annexin A5	35971	4.94	95	2	KqvyEeeY	126–133
38, 39	P02647	Apolipoprotein A-I	30759	5.56	219	5	KwqeEmelY	131–139
42, 43	P06733	Alpha-enolase	47139	7.01	387	5	Rdn.DktrY; KspdDpsrY	50–57; 262–270