Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry

Table 2

Phosphotyrosine-containing proteins identified with 2DGE-based Western blotting and tandem mass spectrometry.

Spot number	Swiss-Prot number	Protein name	Monoisotopic mass of neutral peptide (calc)	Phosphotyrosine-containing peptide sequences	Predicted Tyr-phosphomotif	Tyr-phosphomotif position
3	Q13332	Receptor-type tyrosine-protein phosphatase S	1940.8	GY^*QVHY^*VRMEGAEAR	RtprDenvY; Ksq.Dgp.Y; Rgv.EgsdY	97–105; 370–376; 1728–1735
42	Q13332	Receptor-type tyrosine-protein phosphatase S	1940.8	GY^*QVHY^*VRMEGAEAR	RtprDenvY; Ksq.Dgp.Y; Rgv.EgsdY	97–105; 370–376; 1728–1735
6	Q96P48	Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1	1496.5	(RLDTDHLR)Y^*FDSNKDAY^*SK	RldtDhlrY; RchlEak.Y; Kyr.Egk.Y; Kpl.Ekh.Y	351–359; 637–644; 643–649; 731–737
6	Q8TEP8	Centrosomal protein of 192 kDa	1792.8	KDVLDFGDLTY^*GGWK	Kkt.EvsrY	190–197
12	Q9Y4D7	Plexin-D1	2110.0	(KSRDR)FSY^*VLPLVHSLEPTM^#GPK	Ksr.DrfsY; Kdl.DtekY	971–978; 1666–1673
21	Q9P2D3	HEAT repeat-containing protein 5B	1325.6	M^#AQY^*SFDKLK	RnkdDtaaY; Kal.EnsnY; ReaaEvceY	100–108; 225–232; 1096–1104
21	Q5MCW4	Zinc finger protein 569	1511.6	KEHCEY^*NEPVK	KhtgEkp.Y	655–662
42	P06865	Beta-hexosaminidase subunit alpha	1187.5	EDIPVNY^*MK	Kql.Esf.Y	349–355
46	P49639	Homeobox protein Hox-A1	1967.9	TGKVGEY^*GY^*LGQPNAVR	Kvg.Eyg.Y	219–225
47	Q6P2Q9	Pre-mRNA-processing-splicing factor 8	1325.6	HTLAY^*DKGWR	KfrhDkrvY; RvrtDfkqY	80–88; 1437–1445

Y^* = phosphotyrosine residue, M^# = oxidated methine residue.