New Developments in Peritoneal Fibroblast Biology: Implications for Inflammation and Fibrosis in Peritoneal Dialysis

Figure 1

Expression of FSP-1 by human peritoneal fibroblasts and mesothelial cells. Human peritoneal mesothelial cells and peritoneal resident fibroblasts were isolated from apparently normal omentum by enzymatic digestion, as described [5]. Populations of mesothelial cells and fibroblasts were immunostained for FSP-1 (red) and cytokeratin (green). Nuclei were counterstained with DAPI (blue). Magnification 200x. (a) Human peritoneal fibroblasts express FSP-1 but not cytokeratin. (b) Human peritoneal mesothelial cells express cytokeratin but not FSP-1. (c) Stimulation of mesothelial cells with TGF-β1 (1 ng/mL; 72 hours) leads to mesenchymal transition that is associated with loss of cytokeratin expression and de novo FSP-1 expression.

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