N1303K (c.3909C>G) Mutation and Splicing: Implication of Its c.[744-33GATT(6); 869+11C>T] Complex Allele in CFTR Exon 7 Aberrant Splicing

Figure 2

Impact of the c.3909C>G mutation on exon 24 splicing. Sequences of the cDNA were obtained from transfected cells with the pTBNdeI minigene plasmid carrying (a) WT or (b) c.3909C>G exon 24 CFTR minigenes. In both plasmids the sequencing reveals the same transcript, excluding an impact of the c.3909C>G mutation on splicing (the presence of intronic parts in WT and mutated results from plasmid construction and has been detected in the three cell lines).

(a)

(b)