Effect of Dietary Lead on Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens

Table 2

Primer sequences () used in real-time PCR.

Name	Forward primer	Reverse primer	Product size (bp)	Annealing; Temperature °C
SOD^†	AGGGGGTCATCCACTTCC	CCCATTTGTGTTGTCTCCAA	122	62.1
CAT	GGGGAGCTGTTTACTGCAAG	TTTCCATTGGCTATGGCATT	139	60
GSTα	GCCTGACTTCAGTCCTTGGT	CCACCGAATTGACTCCATCT	131	62.1
Hsp70	AACCGCACCACACCCAGCTATG	CTGGGAGTCGTTGAAGTAAGCG	359	65
IL2	TGCAGTGTTACCTGGGAGAA	CTTGCATTCACTTCCGGTGT	135	60.2
IL6	GACTCGTCCGGAGAGGTTG	CGCACACGGTGAACTTCTT	128	60.2
SGLT1	TGTCTCTCTGGCAAGAACATGTC	GGGCAAGAGCTTCAGGTATCC	229	60.2
SGLT5	ATACCCAAGGTAATAGTCCCAAAC	TGGGTCCCTGAACAAATGAAA	75	60
GLUT2	CACACTATGGGCGCATGCT	ATTGTCCCTGGAGGTGTTGGTG	116	60
GLUT5	TTGCTGGCTTTGGGTTGTG	GGAGGTTGAGGGCCAAAGTC	99	60
PepT1	CCCCTGAGGAGGATCACTGTT	CAAAAGAGCAGCAGCAACGA	205	60
EAAT3	TGCTGCTTTGGATTCCAGTGT	AGCAATGACTGTAGTGCAGAAGTAATATATG	79	60
GAPDH	GCCGTCCTCTCTGGCAAAG	TGTAAACCATGTAGTTCAGATCGATGA	128	60
B-actin	ATGAAGCCCAGAGCAAAAGA	GGGGTGTTGAAGGTCTCAAA	175	60

SOD (superoxide dismutase); CAT (catalase); GST-α (3-glutathione S-transferase-α); Hsp70 (heat shock protein); IL2 (interleukin 2); IL6 (interleukin 6); SGLT1 (Na⁺-dependent glucose and galactose transporter); SGLT5 (glucose transporter); GLUT2 (Na⁺-independent glucose, galactose and fructose transporter); GLUT5 (Na⁺-independent fructose transporter); PepT1 (oligopeptide transporter); EAAT3 (excitatory amino acid transporter 3, Na⁺, H⁺, K⁺ dependent); GAPDH (glyceraldehyde phosphate dehydrogenase).