Research Article
Effect of Dietary Lead on Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens
Table 2
Primer sequences (
) used in real-time PCR.
| Name | Forward primer | Reverse primer | Product size (bp) | Annealing Temperature °C |
| SOD† | AGGGGGTCATCCACTTCC | CCCATTTGTGTTGTCTCCAA | 122 | 62.1 | CAT | GGGGAGCTGTTTACTGCAAG | TTTCCATTGGCTATGGCATT | 139 | 60 | GSTα | GCCTGACTTCAGTCCTTGGT | CCACCGAATTGACTCCATCT | 131 | 62.1 | Hsp70 | AACCGCACCACACCCAGCTATG | CTGGGAGTCGTTGAAGTAAGCG | 359 | 65 | IL2 | TGCAGTGTTACCTGGGAGAA | CTTGCATTCACTTCCGGTGT | 135 | 60.2 | IL6 | GACTCGTCCGGAGAGGTTG | CGCACACGGTGAACTTCTT | 128 | 60.2 | SGLT1 | TGTCTCTCTGGCAAGAACATGTC | GGGCAAGAGCTTCAGGTATCC | 229 | 60.2 | SGLT5 | ATACCCAAGGTAATAGTCCCAAAC | TGGGTCCCTGAACAAATGAAA | 75 | 60 | GLUT2 | CACACTATGGGCGCATGCT | ATTGTCCCTGGAGGTGTTGGTG | 116 | 60 | GLUT5 | TTGCTGGCTTTGGGTTGTG | GGAGGTTGAGGGCCAAAGTC | 99 | 60 | PepT1 | CCCCTGAGGAGGATCACTGTT | CAAAAGAGCAGCAGCAACGA | 205 | 60 | EAAT3 | TGCTGCTTTGGATTCCAGTGT | AGCAATGACTGTAGTGCAGAAGTAATATATG | 79 | 60 | GAPDH | GCCGTCCTCTCTGGCAAAG | TGTAAACCATGTAGTTCAGATCGATGA | 128 | 60 | B-actin | ATGAAGCCCAGAGCAAAAGA | GGGGTGTTGAAGGTCTCAAA | 175 | 60 |
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SOD (superoxide dismutase); CAT (catalase); GST-α (3-glutathione S-transferase-α); Hsp70 (heat shock protein); IL2 (interleukin 2); IL6 (interleukin 6); SGLT1 (Na+-dependent glucose and galactose transporter); SGLT5 (glucose transporter); GLUT2 (Na+-independent glucose, galactose and fructose transporter); GLUT5 (Na+-independent fructose transporter); PepT1 (oligopeptide transporter); EAAT3 (excitatory amino acid transporter 3, Na+, H+, K+ dependent); GAPDH (glyceraldehyde phosphate dehydrogenase).
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