PI3K/AKT/mTOR/p70S6K Pathway Is Involved in Aβ25-35-Induced Autophagy

Figure 2

LC3 immunoreactivity is shown. HT22 cells were stained for LC3 (red arrows) and with the nuclear dye DAPI (blue) following the different treatments for 24 h. (Control) Negative control group: LC3 punctate dots (red) can barely be detected. (10 μM) 10 μM Aβ25-35 group: the dots exhibited a scattered distribution in the perinuclear region. (40 μM) 40 μM Aβ25-35 group: the dots are evenly spread around the perinuclear and cytoplasm regions. (RP) rapamycin group. (40 μM + Baf) 40 μM Aβ25-35 and bafilomycin A1 (Baf, 100 nM) group: the cells are filled with cluster-like dots. .

(a)

(b)