Proregenerative Microenvironment Triggered by Donor Mesenchymal Stem Cells Preserves Renal Function and Structure in Mice with Severe Diabetes Mellitus

Figure 9

MSC administration prevents renal inflammation. Representative photomicrographs of kidney sections showing inflammatory infiltrates (white arrow) eight weeks after MSC administration. Bar = 100 μm (a). Representative photomicrographs of kidney sections showing macrophages (white arrows) (F4/80-red) eight weeks after MSC administration. Barr = 40 μm (b). Nuclei were counterstained with DAPI (blue). Quantitative analysis of macrophages per tissue section eight weeks after MSC administration (c). Quantitative analysis of plasmatic levels of the anti-inflammatory cytokines IL-6 (d), IL-10 (e), and IL-4 (f) determined by the Luminex Multiplex system two and eight weeks after MSC administration. Renal mRNA levels of IL-10 (g) and IL-4 (h) quantified by qRT-PCR, two and eight weeks after MSC administration. Data are presented as mean ± SEM, , versus Normal; versus DM for the same time point.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)